Invitrogen bn-page manual

The Invitrogen NuPAGE Antioxidant running buffer additive greatly minimizes protein oxidation during electrophoresis and keeps reduced protein bands sharp and clear. High sample integrity. Integrity of samples is maintained throughout electrophoresis with the NuPAGE Bis-Tris Gel System (left), compared to samples prepared with Laemmli (tris-glycine) sample buffer (right). In standard SDS-PAGE, the charge-shift molecule is SDS. The SDS denatures proteins and binds to proteins conferring a net negative charge allowing the proteins to migrate in one direction towards the anode. The SDS is present in the sample buffer and running buffer. In BN PAGE, the Coomassie G binds to proteins and confers a net negativeFile Size: KB. Polyacrylamide Gel Electrophoresis (PAGE) 10 Discontinuous Native PAGE 10 SDS-PAGE 11 Other Types of PAGE 12 Blue Native PAGE (BN-PAGE) 12 Zymogram PAGE 12 Isoelectric Focusing (IEF) 12 2-D Electrophoresis 13 Electrophoresis Cells and Power Supplies 13 Electrophoresis Cells 13 Power Supplies for PAGE Applications 15 Chapter 3 Sample .

NativePAGE™ 5% G Sample Additive is added to samples prepared with the NativePAGE™ Sample Buffer and detergents (10% DMM or 5% Digitonin) just prior to applying the samples to NativePAGE™ gels. This Additive is also included in the NativePAGE™ Sample Prep Kit. The samples prepared with 10% DDM, 5% Digitonin, or the NativePAGE. Invitrogen™ CellEvent™ Caspase-3/7 Green Detection Reagent. CellEvent™ Caspase-3/7 Green Detection Reagent is a novel fluorogenic substrate for activated caspase-3/7 that is compatible with both live cell and fixed imaging, with absorption/emission maxima of ∼/ nm. The gels do not contain any G This system, based on the blue native polyacrylamide gel electrophoresis (BN PAGE) technique developed by Schägger and von Jagow, overcomes the limitations of traditional native gel electrophoresis by providing a near-neutral operating pH and detergent compatibility.

Blue-Native Polyacrylamide Gel Electrophoresis (BN PAGE) that overcomes the limitations of traditional Invitrogen now introduces NativePAGE™ Novex®. Mar While BN-PAGE retains the native state of proteins, it falls short were subjected to SDS-PAGE using the protocol provided by Invitrogen. Blue native polyacrylamide gel electrophoresis (BN–PAGE) is an essential tool for investigating height of the individual bands (NativeMark, Invitrogen).